Generational sensitivity alteration in Chironomus yoshimatsui to carbamate and pharmaceutical chemicals and the effect on Catalase, CYP450, and hemoglobin gene transcription

To ascertain the tolerance mechanisms of aquatic organisms to artificial chemicals, intergenerational sensitivity changes Chironomus yoshimatsui a carbamate pesticide (pirimicarb) and pharmaceutical chemical (diazepam) were investigated. The larvae (<48-h-old) in each generation exposed both chemicals for 48 h then surviving chironomids cultured until fifth (F0–F4) without addition. 48-h 50% effective concentration (EC50) value was determined generation. In pirimicarb treatment group, EC50 values significantly increased F3 F4, those diazepam group slightly increased. Catalase, Cytochrome P450 hemoglobin (Hb) mRNA levels monitored see whether these related trans-generational sensitivity. Although generalized linear model results showed that treatment, we could not find any alteration. contrast, approach trans-generationally became tolerant with increasing Hb levels. Therefore, they might decrease their stress by modifying gene transcription.